Democratizing single molecule nanoarrays

Abstract: Arrays of individual molecules or nanoparticles can combine the advantage of microarrays and single-molecule experiments. They facilitate miniaturized high-throughput assays with low sample and reagent consumption while also revealing heterogeniety hidden in ensemble measurements. However, creating single molecule arrays are particularly difficult since conventional nano-fabrication techniques can't be used to organize individual molecules onto defined positions on a substrate. In this context 'DNA origami placement' is an appealing technique as it allows any molecule (or nanoparticle) that can be labeled with DNA to be organized on arbitrary substrates using standard lithographic processes. The challenge now becomes one of reducing cost, developing applications, and increasing the capabilities.

In this talk, Gopinath will introduce a bench-top technique to create cm-scale DNA origami nanoarrays that does not require any nanopatterning step, at a cost of $1 per cm2 and Gopinath will also discuss the application of this technique for point-of-care diagnostics. Finally, Gopinath will end by introducing strategies for performing non-aqueous organic reaction on immobilized DNA origami for creating arrays of extremely hydrophobic molecules.

BiologyComputer sciencePhysics

Audience: general audience

SENSE.nano Symposium from MIT.nano